A.D.N Rupidara


The cross-streak method was employed to determine the potential biocontrol properties of bacterial isolates from healthy corals. t was used to test cross with bacteria that were isolated from the diseased coral of A. millepora species and also A. aspera species. Of the nine isolates of the healthy A. millepora(Ehrenberg, 1834) four (SUM C, SUM F, SUM G and SUM H) showed antagonistic effect over the remaining isolates. These together with the eight isolates of A. aspera (SUA A, SUA E, SUA H, SUA J, SUA L, SUA O, SUA T and SUA V) were tested for their biocontrol potential. Only two of the four isolates from healthy A. millepora inhibited the growth of bacterial isolates from the diseased A. millepora , one (SUM F) inhibiting 100% and one ( SUM C) 85%. On the other hand, for the isolates of A. aspera, SUA V inhibited 50% of the diseased isolates while SUA J performed poorly and was inhibited by all the isolates from the diseased A. aspera. Generally, bacterial isolates from healthy corals can be a potential biocontrol.

Keywords: biocontrol potential, coral associated bacteria, mucus, healthy coral, diseased coral.

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Ben-Haim, Y, & Rosenberg, E. (2002).A novel Vibrio sp. pathogen of the coral Pocilloporadamicornis.Marine Biology, Vol. 141, 1, pp 47-55.

Ben-Haim, Y., Banim, E., Kushmaro, A., Loya, Y., & Rosenberg, E. (1999).Inhibition of photosynthesis and bleaching of zooxanthellae by the coral pathogen Vibrio shiloi.Environmental Microbiology (1999) 1(3), 223-229.

Brown, B. E. (1997). Coral bleaching: causes and consequences. Coral Reefs 16 (suppl.):S129–S138.

Di Salvo, L.H, &Gundersen, K. (1971). Regenerative funcions and microbial ecology of coral reefs Part I: Assay for microbial population. Can J. Microbial 17: 100081-9 in O. A. Jones & R. Endean, (1973). Biology and geology of coral reefs. Vol. II. (p. 1 – 15). New York: Academic Press.

Ducklow H.W., & Mitchell, R. (1979).Bacterial populations and adaptations in mucus layers of living corals.Limnol.Oceanogr. 24, 715-725.

Glynn, P. W. (1993). Coral reef bleaching: ecological perspectives. Coral Reefs 12:1–17.

Handelsman‘, J., &Stabb, E.V. (1996).Biocontrol of Soilborne Plant Pathogens.The Plant Cell, Vol. 8, 1855-1869, October 1996.

Hoegh-Guldberg, O. (1999). Climate change, coral bleaching and the future of the world’s coral reefs.Marine and Freshwater Research, 50, 839–866.

Knowlton, N., &Rohwer, F., (2003). Multispecies Microbial Mutualisms on Coral Reefs: The Host as a Habitat.Supplement the American Naturalist, 162, October 2003, S51–S62.

Koren, O., & Rosenberg, E. (2006). Bacteria Associated with mucus and tissues of the coral Oculinapatagonicain Summer and Winter. Applied And Environmental Microbiology, 72, 8, 5254 – 5259. doi:10.1128/AEM.00554-06.

Nissimov, J., Rosenberg, E., & Munn, C.B. (2009).Antimicrobial properties or resident coral mucus bacteria of Oculinapatagonica.Research letter.FEMS MirobiolLett 292, 210-215.

Ritchie, K.B. (2006). Regulation of microbial populations by coral surface mucus and mucus-associated bacteria.Mar EcolProgSer, 322, 1–14.

Rohwer, F., Seguritan, V., Azam, F., & Knowlton, N. ( 2002). Diversity and distribution of coral-associated bacteria.Mar EcolProgSer 243, 1–10.

Rohwer, F., Breitbart M., Jara J., Azam F., & Knowlton N., (2001). Diversity of bacteria associated with Caribbean coral Montastraeafranksi. Coral Reef 20, 85-91.

Rosenberg, E., Koren, O., Reshef, L., Efrony, R., &Zilber-Rosenberg, I. (2007a,).The role of microorganisms in coral health, disease and evolution.Nature reviews Microbiology (5), 355-362.

Rosenberg, E., Kellogg, C.A., &Rohwer, F. (2007b).Coral microbiology.Oceanography 20.2, 146 - 154.

Shnit-Orland, M., &Kushmaro, A. (2009). Coral mucus-associated bacteria: a possible first line of defense. FEMS Microbiol Ecol., 67, 371–380. doi:10.1111/j.1574-6941.2008.00644.x

Zhang, Y.Y., Ling, J., Yang, Q.S., Wang, Y.S., Sun, C.C., Sun, H.Y., Feng, J.B., Jiang, Y.F., Zhang, Y.F., Wu, M.L., & Dong, J.D. (2015) The diversity of coral associated bacteria and the environmental factors affect their community variation. Ecotoxicology. 2015 Oct; 24, (7-8), 1467-77. doi: 10.1007/s10646-015-1454-4.